Fli1^tm1Dkw
Targeted Allele Detail

Summary

• Symbol: Fli1^tm1Dkw
• Name: Friend leukemia integration 1; targeted mutation 1, Dennis K Watson
• MGI ID: MGI:2386167
• Gene: Fli1 Location: Chr9:32333500-32454157 bp, - strand Genetic Position: Chr9, 17.74 cM
• Alliance: Fli1^tm1Dkw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63242
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A floxed neo cassette was inserted into exon 9 via homologous recombination resulting in a premature stop codon between the DNA-binding domain and the CTA domain. The targeted mutation results in a hypomorphic allele. Northern blot analysis of homozygous mutant embryos showed 2 gene transcripts at 10% the level of wild-type transcription. Western blot analysis detected a truncated protein expressed at very low levels. Enzyme assay determined that the truncated protein exhibits 50%-60% of the activity of normal protein. (J:63242)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fli1 Mutation: 26 strains or lines available

Notes

TC1-10 ES cells were used.

References

• Original: J:63242 Spyropoulos DD, et al., Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol Cell Biol. 2000 Aug;20(15):5643-52
• All: 19 reference(s)