Nup50^tm1Clur
Targeted Allele Detail

Summary

• Symbol: Nup50^tm1Clur
• Name: nucleoporin 50; targeted mutation 1, Bruce E Clurman
• MGI ID: MGI:2386173
• Synonyms: Nup50^-
• Gene: Nup50 Location: Chr15:84807612-84827164 bp, + strand Genetic Position: Chr15, 40.25 cM
• Alliance: Nup50^tm1Clur page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63241
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-4 were replaced with a betageo cassette via homologous recombination. The targeted mutation results in splicing of exon 1 to the betageo cassette which introduces stop codons in all reading frames. Homozygous mutant embryos were detected up to day E18.5 and did not express Nup50 protein as determined by Western blot analysis. (J:63241)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nup50 Mutation: 19 strains or lines available

References

• Original: J:63241 Smitherman M, et al., Characterization and targeted disruption of murine Nup50, a p27(Kip1)-interacting component of the nuclear pore complex. Mol Cell Biol. 2000 Aug;20(15):5631-42
• All: 2 reference(s)