Upf1^tm1Hcd
Targeted Allele Detail

Summary

• Symbol: Upf1^tm1Hcd
• Name: UPF1 RNA helicase and ATPase; targeted mutation 1, Harry C Dietz
• MGI ID: MGI:2386175
• Synonyms: Rent^-
• Gene: Upf1 Location: Chr8:70784175-70805928 bp, - strand Genetic Position: Chr8, 34.15 cM
• Alliance: Upf1^tm1Hcd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67076
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4 through 6 were replaced with a neomycin selection cassette. Northern blot analysis of MEF cell lines derived from heterozygous mutant mice showed that aberrant transcripts were produced from the targeted allele. RT-PCR and sequencing analysis of the these transcripts identified chimeric transcripts that contained the neo gene as well as splice variants in which exon 3 joined exon 8. In either case, a premature stop codon was introduced immediately downstream of exon 3. (J:67076)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Upf1 Mutation: 56 strains or lines available

References

• Original: J:67076 Medghalchi SM, et al., Rent1, a trans-effector of nonsense-mediated mRNA decay, is essential for mammalian embryonic viability. Hum Mol Genet. 2001 Jan 15;10(2):99-105
• All: 1 reference(s)