Pnp^tm1Cmr
Targeted Allele Detail

Summary

• Symbol: Pnp^tm1Cmr
• Name: purine-nucleoside phosphorylase; targeted mutation 1, Chaim M Roifman
• MGI ID: MGI:2386195
• Synonyms: PNP^-
• Gene: Pnp Location: Chr14:51181760-51190869 bp, + strand Genetic Position: Chr14, 26.31 cM, cytoband B-C1
• Alliance: Pnp^tm1Cmr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62870
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.2 kb region of the gene spanning exons 3 and 4, including the catalytic site, was replaced with a PGK-neo cassette via homologous recombination. Homozygous mutant animals were identified and confirmed by Southern blot analysis. (J:62870)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pnp Mutation: 29 strains or lines available

Notes

ES cell line = "E129/Cj."

References

• Original: J:62870 Arpaia E, et al., Mitochondrial basis for immune deficiency. Evidence from purine nucleoside phosphorylase-deficient mice. J Exp Med. 2000 Jun 19;191(12):2197-208
• All: 3 reference(s)