Mesp2^tm3(cre)Ysa
Targeted Allele Detail

Summary

• Symbol: Mesp2^tm3(cre)Ysa
• Name: mesoderm posterior 2; targeted mutation 3, Yumiko Saga
• MGI ID: MGI:2386202
• Gene: Mesp2 Location: Chr7:79460475-79463179 bp, + strand Genetic Position: Chr7, 45.18 cM
• Alliance: Mesp2^tm3(cre)Ysa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62984
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Mesp2^tm3(cre)Ysa expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Mesp2 (MGI:1096325)

• Mutation details: The entire Mesp2 gene was replaced with an in-frame cre gene and a PGK-puro cassette by homologous recombination into an ES cell line containing a disrupted Mesp1 allele (Mesp1^tm3Ysa). This allele expresses Cre recombinase under the control of the Mesp2 promoter. (J:62984)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Mesp2 (mouse) Summary of all recombinase alleles driven by Mesp2. Alliance mouse Mesp2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mesp2 Mutation: 30 strains or lines available

Notes

Chimera analysis in which double homozygous Mesp1^tm3Ysa and Mesp2^tm3(cre)Ysa mutant embryos were aggregated with wild-type embryos show that embryos develop normally, however double knock-out cells are scarcely seen in the anterior-cephalic and heart mesoderm, but they do contribute to the formation of somites, notochord, and gut, and the atrial appendage is exclusively composed of the mutant cells (J:62984).

References

• Original: J:62984 Kitajima S, et al., MesP1 and MesP2 are essential for the development of cardiac mesoderm. Development. 2000 Aug;127(15):3215-26
• All: 2 reference(s)