Gja1^tm2(Gjb1)Kwi
Targeted Allele Detail

Summary

• Symbol: Gja1^tm2(Gjb1)Kwi
• Name: gap junction protein, alpha 1; targeted mutation 2, Klaus Willecke
• MGI ID: MGI:2386240
• Synonyms: Cx43KI32
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm2(Gjb1)Kwi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65112
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Gja1^tm2(Gjb1)Kwi expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gjb1 (MGI:95719)

• Mutation details: The endogenous gene was deleted and replaced with Gjb1 genomic sequence extending from 158 bp upstream of the start codon to 171 bp downstream of the stop codon. RT-PCR verified the ablation of endogenous expression and showed that the Gjb1 sequence was transcribed. The absence of endogenous protein was demonstrated by Western blot ananlysis of brain tissue, which also confirmed the presence of the Gjb1 protein. (J:65112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 60 strains or lines available

References

• Original: J:65112 Plum A, et al., Unique and shared functions of different connexins in mice. Curr Biol. 2000 Sep 21;10(18):1083-91
• All: 9 reference(s)