Rasgrp1^tm1Jstn
Targeted Allele Detail

Summary

• Symbol: Rasgrp1^tm1Jstn
• Name: RAS guanyl releasing protein 1; targeted mutation 1, James C Stone
• MGI ID: MGI:2386261
• Synonyms: RasGRP1^-
• Gene: Rasgrp1 Location: Chr2:117110464-117173358 bp, - strand Genetic Position: Chr2, 59.19 cM
• Alliance: Rasgrp1^tm1Jstn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65115
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P3/J

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 2 was disrupted by the insertion of a cassette containing both Beta-galactosidase and neomycin resistance genes. While the translation of a fusion protein containing residues 1 through 16 of the endogenous joined to the Beta-galactosidase protein was expected, such a polypeptide was undetected by Western blot analysis of thymus and spleen extracts obtained from homozygous mutant mice. (J:65115)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasgrp1 Mutation: 57 strains or lines available

Notes

ES cell line = NX1.

References

• Original: J:65115 Dower NA, et al., RasGRP is essential for mouse thymocyte differentiation and TCR signaling. Nat Immunol. 2000 Oct;1(4):317-21
• All: 42 reference(s)