C3^tm1Pkna
Targeted Allele Detail

Summary

• Symbol: C3^tm1Pkna
• Name: complement component 3; targeted mutation 1, Marcela Pekna
• MGI ID: MGI:2386266
• Synonyms: C3-, C3^tm1Pkny
• Gene: C3 Location: Chr17:57510970-57535136 bp, - strand Genetic Position: Chr17, 29.72 cM, cytoband E1-E3
• Alliance: C3^tm1Pkna page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45857
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 3.5 kb of the gene, a region including exon 24, was replaced with a neomycin resistance cassette via homologous recombination. Western blot analysis showed an absence of C3 beta-chain protein in the serum of homozygous mutant animals. Similarly, ELISA assay did not detect C3 protein in serum from homozygous animals. (J:45857)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C3 Mutation: 103 strains or lines available

References

• Original: J:45857 Pekna M, et al., Targeted disruption of the murine gene coding for the third complement component (C3). Scand J Immunol. 1998 Jan;47(1):25-9
• All: 13 reference(s)