Ikbkb^tm1Ver
Targeted Allele Detail

Summary

• Symbol: Ikbkb^tm1Ver
• Name: inhibitor of kappaB kinase beta; targeted mutation 1, Inder M Verma
• MGI ID: MGI:2386269
• Synonyms: IKK2^-
• Gene: Ikbkb Location: Chr8:23149228-23196605 bp, - strand Genetic Position: Chr8, 11.42 cM, cytoband A3-A4
• Alliance: Ikbkb^tm1Ver page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54323
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two exons encoding amino acids 36-106 of the kinase domain were replaced with a PGK-neo cassette via homologous recombination. The targeted mutation destroys lyine 44, a residue necessary for kinase activity. Homozygous mutant embryos were detected by PCR genotype analysis up to day E14.5. Sequence analysis of RT-PCR products from mouse embryonic fibroblasts (derived from E12.2 homozygous mutant embryos) revealed an in-frame deletion from nucleotides 106-318. However, Western blot analysis using antibodies directed against the C-terminus of Ikbkb failed to detect protein product. (J:54323)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikbkb Mutation: 56 strains or lines available

References

• Original: J:54323 Li Q, et al., Severe liver degeneration in mice lacking the IkappaB kinase 2 gene [see comments]. Science. 1999 Apr 9;284(5412):321-5
• All: 6 reference(s)