Tyrobp^tm1Lll
Targeted Allele Detail

Summary

• Symbol: Tyrobp^tm1Lll
• Name: TYRO protein tyrosine kinase binding protein; targeted mutation 1, Lewis L Lanier
• MGI ID: MGI:2386277
• Synonyms: DAP12^-
• Gene: Tyrobp Location: Chr7:30113207-30117007 bp, + strand Genetic Position: Chr7, 17.45 cM, cytoband B
• Alliance: Tyrobp^tm1Lll page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64763
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 were deleted by the insertion of a floxed neo cassette, which was subsequently excised via the in vitro expression of cre recombinase. The targeted region encoded the transmembrane domain and a portion of the cytoplasmic domain including the first tyrosine of the immunoreceptor tyrosine-based activation motif (ITAM). The ablation of protein expression in homozygous mutant mice was confirmed via immunohistochemical analysis of spleen sections. (J:64763)

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tyrobp Mutation: 12 strains or lines available

References

• Original: J:64763 Bakker AB, et al., DAP12-deficient mice fail to develop autoimmunity due to impaired antigen priming. Immunity. 2000 Sep;13(3):345-53
• All: 50 reference(s)