Adcyap1r1^tm1Aba
Targeted Allele Detail

Summary

• Symbol: Adcyap1r1^tm1Aba
• Name: adenylate cyclase activating polypeptide 1 receptor 1; targeted mutation 1, Akemichi Baba
• MGI ID: MGI:2386283
• Synonyms: exon 2^-
• Gene: Adcyap1r1 Location: Chr6:55428963-55478436 bp, + strand Genetic Position: Chr6, 27.38 cM, cytoband B3
• Alliance: Adcyap1r1^tm1Aba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65199
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The insertion of a neomycin selection cassette disrupted exon 2, which encodes the 5' untranslated region, the start codon, and the majority of the signal peptide. Northern blot and RT-PCR analyses identified an aberrant transcript in which exon 1 had spliced to exon 3. In vitro studies of the protein generated from a start codon in exon 3 of this transcript showed improper localization and post-translational processing. Specifically, cell surface expression of the mutant protein was reduced and it was largely unglycosylated. (J:65199)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adcyap1r1 Mutation: 34 strains or lines available

References

• Original: J:65199 Hashimoto H, et al., Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptide. J Neurochem. 2000 Nov;75(5):1810-7
• All: 3 reference(s)