H2-Oa^tm1Dma
Targeted Allele Detail

Summary

• Symbol: H2-Oa^tm1Dma
• Name: histocompatibility 2, O region alpha locus; targeted mutation 1, Daniel M Altmann
• MGI ID: MGI:2386294
• Synonyms: H2-Oa-
• Gene: H2-Oa Location: Chr17:34311314-34314208 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Oa^tm1Dma page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:65235
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence extending from nucleotide position 21 in exon 1 through position 861 in exon 5 was replaced with a cassette containing lacZ and neo via homologous recombination. Transcript was undetected in homozygous mutant mice via RT-PCR analysis of total spleen and thymis RNA. Homozygous mutant mice were shown to lack the endogenous protein via Western blot analysis of splenocytes. (J:65235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H2-Oa Mutation: 11 strains or lines available

References

• Original: J:65235 Perraudeau M, et al., Altered major histocompatibility complex class II peptide loading in H2-O-deficient mice. Eur J Immunol. 2000 Oct;30(10):2871-80
• All: 1 reference(s)