Igf1r^tm1Mhz
Targeted Allele Detail

Summary

• Symbol: Igf1r^tm1Mhz
• Name: insulin-like growth factor I receptor; targeted mutation 1, Martin Holzenberger
• MGI ID: MGI:2386327
• Synonyms: IGF-IRneo
• Gene: Igf1r Location: Chr7:67602575-67883416 bp, + strand Genetic Position: Chr7, 37.27 cM
• Alliance: Igf1r^tm1Mhz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63460
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 3 was flanked by a floxed neo cassette inserted into intron 2 and a single loxP site in intron 3. Several aberrant transcripts involving the splicing of exon 2 to cryptic slice sites in the neo transgene were identified by RT-PCR and sequence analyses. Further analysis indicated that the readthrough of the neo transgene will result in functional ablation due to truncation or the inclusion of a random neo fragment. Mice carrying this allele were crossed with transgenic mice expressing cre recombinase via the EIIa promoter to generate Igf1r^tm1.1Mhz and Igf1r^tm1.2Mhz. (J:63460)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igf1r Mutation: 88 strains or lines available

References

• Original: J:63460 Holzenberger M, et al., A targeted partial invalidation of the insulin-like growth factor I receptor gene in mice causes a postnatal growth deficit. Endocrinology. 2000 Jul;141(7):2557-66
• All: 6 reference(s)