Epas1^tm1Fong
Targeted Allele Detail

Summary

• Symbol: Epas1^tm1Fong
• Name: endothelial PAS domain protein 1; targeted mutation 1, Guo-Hua Fong
• MGI ID: MGI:2386334
• Synonyms: EPAS-1
• Gene: Epas1 Location: Chr17:87061292-87140838 bp, + strand Genetic Position: Chr17, 56.9 cM
• Alliance: Epas1^tm1Fong page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63415
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The majority of exon 2, which encodes the basic helix-loop-helix domain, was replaced with a cassette containing both IRES-lacZ and neo genes. Transcript was undetected by RT-PCR analyses of homozygous mutant embryos. Weak expression of beta-galactosidase, putatively due to the inefficiency of the IRES, showed the pattern of expression to recapitulate that of the endogenous gene. (J:63415)

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epas1 Mutation: 66 strains or lines available

References

• Original: J:63415 Peng J, et al., The transcription factor EPAS-1/hypoxia-inducible factor 2alpha plays an important role in vascular remodeling. Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8386-91
• All: 5 reference(s)