Gal3st1^tm1Kho
Targeted Allele Detail

Summary

• Symbol: Gal3st1^tm1Kho
• Name: galactose-3-O-sulfotransferase 1; targeted mutation 1, Koichi Honke
• MGI ID: MGI:2386400
• Synonyms: Cst^-, CST^-, CST^null
• Gene: Gal3st1 Location: Chr11:3933636-3949326 bp, + strand Genetic Position: Chr11, 2.81 cM, cytoband A1
• Alliance: Gal3st1^tm1Kho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78560
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 1,126 bp of the gene, including part of exon 2 and part of exon 3, was replaced with a PGK-neo cassette via homologous recombination. The targeted mutation disrupts the transmembrane domain and the 5'-3' phosphoadenosine 5'-phosphosulfate-binding motif. Northern blot analysis of brain RNA and RT-PCR analysis of testicular RNA showed that gene expression is abolished in homozygous mutant animals. (J:78560)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gal3st1 Mutation: 22 strains or lines available

References

• Original: J:78560 Honke K, et al., Paranodal junction formation and spermatogenesis require sulfoglycolipids. Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4227-32
• All: 18 reference(s)