Spam1^tm1Tba
Targeted Allele Detail

Summary

• Symbol: Spam1^tm1Tba
• Name: sperm adhesion molecule 1; targeted mutation 1, Tadashi Baba
• MGI ID: MGI:2386404
• Synonyms: Ph20^-
• Gene: Spam1 Location: Chr6:24791187-24801047 bp, + strand Genetic Position: Chr6, 11.28 cM, cytoband A2
• Alliance: Spam1^tm1Tba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78559
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2, which encodes the hyaluronidase domain, was replaced with a neomycin resistance cassette via homologous recombination. Testicular RNA from homozygous mutant animals did not contain gene transcripts as assayed by Northern blot. Western blot of cauda epididymal sperm protein extracts from homozygous mutants showed no immunoreactivity. (J:78559)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Spam1 Mutation: 32 strains or lines available

References

• Original: J:78559 Baba D, et al., Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg. J Biol Chem. 2002 Aug 16;277(33):30310-4
• All: 7 reference(s)