Kif3a^tm2Gsn
Targeted Allele Detail

Summary

• Symbol: Kif3a^tm2Gsn
• Name: kinesin family member 3A; targeted mutation 2, Lawrence SB Goldstein
• MGI ID: MGI:2386464
• Synonyms: Kif3a^fl, KIF3A^flox, Kif3a^floxed, Kif3a^tm2Maz
• Gene: Kif3a Location: Chr11:53458206-53492794 bp, + strand Genetic Position: Chr11, 31.97 cM, cytoband A5-B1
• Alliance: Kif3a^tm2Gsn page

Polycystic kideny disease in Kif3a^tm1Gsn/Kif3a^tm2Gsn Tg(Cdh16-cre)91Igr/0 mutant mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63427
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A floxed Tk-Neo cassette was inserted in intron 1 and a third loxP site was inserted downstream of exon 2. Successfully targeted ES cells were transiently transfected with a cre expressing vector which excised the floxed selection cassette. Exon 2 was left flanked by single intronic loxP sites. Western blot analysis of retina and brain tissues showed protein expression to be unaffected by the presence of the loxP sites. This allele was used to generate Kif3a^tm1Gsn. (J:54575, J:63427, J:131271)

Constructon of the Kif3a^tm2Gsn allele

Disease models

Expression

In Mice Carrying this Mutation:	13 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	35 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kif3a Mutation: 33 strains or lines available

References

• Original: J:63427 Marszalek JR, et al., Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors. Cell. 2000 Jul 21;102(2):175-87
• All: 82 reference(s)