Polb^tm1Rsky
Targeted Allele Detail

Summary

• Symbol: Polb^tm1Rsky
• Name: polymerase (DNA directed), beta; targeted mutation 1, Klaus Rajewsky
• MGI ID: MGI:2386535
• Synonyms: BT14, PolBeta^flox, PolB^flox, Polb^tm1Hua
• Gene: Polb Location: Chr8:23118142-23143451 bp, - strand Genetic Position: Chr8, 11.42 cM
• Alliance: Polb^tm1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67942
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Single loxP sites were left flanking exon 1 and 2 kb of upstream sequence including the promoter after a neomycin selection cassette was excised via the in vitro expression of cre recombinase. (J:67942)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Polb Mutation: 28 strains or lines available

References

• Original: J:67942 Gu H, et al., Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science. 1994 Jul 1;265(5168):103-6
• All: 11 reference(s)