Tg(Nes-cre)1Wme
Transgene Detail

Summary

• Symbol: Tg(Nes-cre)1Wme
• Name: transgene insertion 1, Werner Muller
• MGI ID: MGI:2386545
• Synonyms: bal1, Bal1 cre, balancer1, Nestin-Cre, SLNE4c, Tg(balancer1)2Cgn, TgN(balancer1)1Cgn, TgN(balancer1)2Cgn
• Transgene: Tg(Nes-cre)1Wme Location: unknown
• Alliance: Tg(Nes-cre)1Wme page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Nes-cre)1Wme expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Nes

• Mutation details: The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included upstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of loxP-flanked alleles occurs before embryonic day 10.5, and it is detectable in all adult organs examined including germ line cells. (J:67926)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nes (species not specified) Summary of all recombinase alleles driven by Nes. Comparative matrix view of recombinase activities

Expression

In Mice Carrying this Mutation:	98 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Mice carrying this "balancer 1" transgene show less variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 2" transgene.

References

• Original: J:67926 Betz UA, et al., Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Curr Biol. 1996 Oct 1;6(10):1307-16
• All: 63 reference(s)