Tg(RP23-291P1)1Flp
Transgene Detail

Summary

• Symbol: Tg(RP23-291P1)1Flp
• Name: transgene insertion 1, Figge, Lammert and Paigen
• MGI ID: MGI:2386569
• Synonyms: Abcb11-BAC, F46, Tg(Abcb11)1, Tg(RP23-291P1)46Flp, TTR-Abcb11
• Transgene: Tg(RP23-291P1)1Flp Location: unknown
• Alliance: Tg(RP23-291P1)1Flp page

Transgene origin

• Strain of Origin: 129S1/SvImJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(RP23-291P1)1Flp expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Abcb11 (MGI:1351619)
  mouse	G6pc2 (MGI:1277193)

• Mutation details: The transgene comprises one to two copies of bacterial artificial chromosome (BAC) clone RP23-291P1, whose 182-kb, C57BL/6J-derived genomic DNA insert contains the Abc11 gene, 33 kb of 5' flanking and 44 kb of 3' flanking DNA, and the entire glucose-6-phosphatase, catalytic, 2 (G6pc2) gene. Northern blot analysis of total liver RNA and western blot analysis of canalicular liver plasma membrane extracts showed about two-fold elevation of Abc11 transcript and protein levels in transgenic mice fed a chow diet; transcript levels were unaffected by consumption of a lithogenic diet for 8 weeks. (J:94496)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The primary publication does not distinguish, in describing experimental results, between this and another transgenic line, designated Tg(RP23-291P1)2Flp, generated in the same strain (129S1/SvImJ) using this BAC.

Some differences, though not statistically significant, were noted in semi-quantitative scores of gallbladder biles of transgenic vs. wild-type 129S1/SvImJ mice fed a lithogenic diet for 4 weeks for:

• cholesterol monohydrate crystals (1.4 +/- 0.6 vs. 1.1 +/- 0.6)

• sandy stones (0.5 +/- 0.3 vs. 0.4 +/- 0.3)

• prevalence of true gallstones (25% vs. 14%)

Densitometric analysis of northern blots of total liver RNA from chow-fed mice revealed Cyp7a1 expression of transgenic mice to be only 35% +/- 14% that in wildtype mice.

References

• Original: J:94496 Figge A, et al., Hepatic overexpression of murine Abcb11 increases hepatobiliary lipid secretion and reduces hepatic steatosis. J Biol Chem. 2004 Jan 23;279(4):2790-9
• All: 4 reference(s)