Vegfa^tm3.1Pec
Targeted Allele Detail

Summary

• Symbol: Vegfa^tm3.1Pec
• Name: vascular endothelial growth factor A; targeted mutation 3.1, Peter Carmeliet
• MGI ID: MGI:2386593
• Synonyms: VEGF¹⁶⁴
• Gene: Vegfa Location: Chr17:46327919-46343295 bp, - strand Genetic Position: Chr17, 22.79 cM
• Alliance: Vegfa^tm3.1Pec page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75507
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Intragenic deletion
• Mutation details: The floxed neo cassette was excised from Vegfa^tm3Pec by the in vivo expression of cre recombinase, leaving the isoform specific cDNA replacement intact. The resultant allele lacks exon 6 as well as splice sites and thereby produces a single isoform with intermediate solubility and receptor binding properties. The exclusive presence of this isoform was verified by quatitative RT-PCR. (J:75507)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Vegfa Mutation: 38 strains or lines available

References

• Original: J:75507 Stalmans I, et al., Arteriolar and venular patterning in retinas of mice selectively expressing VEGF isoforms. J Clin Invest. 2002 Feb;109(3):327-36
• All: 9 reference(s)