Fmn1^tm1Led
Targeted Allele Detail

Summary

• Symbol: Fmn1^tm1Led
• Name: formin 1; targeted mutation 1, Philip Leder
• MGI ID: MGI:2386598
• Synonyms: fmn^4KO
• Gene: Fmn1 Location: Chr2:113158081-113547112 bp, + strand Genetic Position: Chr2, 57.3 cM, cytoband C1-qter
• Alliance: Fmn1^tm1Led page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50647
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 was replaced by a PGK-neo cassette inserted by homologous recombination. Circular transcripts were undetected by RT-PCR analysis of brain and kidney samples obtained from homozygous mutant mice. Ribonuclease protection assays showed that transcripts lacking the targeted exon were present at normal levels in homozygous mutant mice. Corresponding results were obtained by Western blot analysis. (J:50647)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fmn1 Mutation: 76 strains or lines available

Notes

Homozygous mutant mice have normal limbs, but display variably penetrant renal aplasia characteristic of other limb deformity mutant alleles (J:50647).

References

• Original: J:50647 Chao CW, et al., The mouse formin (Fmn) gene: abundant circular RNA transcripts and gene-targeted deletion analysis. Mol Med. 1998 Sep;4(9):614-28
• All: 1 reference(s)