Lhyp^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Lhyp^C57BL/6J
• Name: lung hyperpermeability; C57BL/6J
• MGI ID: MGI:2386632
• QTL: Lhyp Location: Chr4:60454011-66764818 bp Genetic Position: Chr4, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers susceptibility to ozone-induced lung hyperpermeability compared to C3H/HeJ. (J:66468)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:66468

12 C57BL/6J x C3H/HeJ (BXH) recombinant inbred (RI) strains were screened for 558 SSLPs to identify QTLs associated with susceptibility to ozone-induced lung hyperpermeability. Parental strain C57BL/6J is susceptible to ozone-induced injury and exhibits greater lung hyperpermeability compared to the resistant parental strain C3H/HeJ. A locus on mouse Chromosome 4, Lhyp, was identified with significant linkage to ozone-induced lung hyperpermeability (LRS=16.6) and maps to an interval between Mup1 (27.8 cM) and Tlr4 (33 cM). Suggestive loci were detected on distal chromosome 3 and central chromosome 11. Since the Tlr4 allele differs between C57BL/6J (wild type) and C3H/HeJ (mutant), this gene was investigated as a candidate for Lhyp. Substrain C3H/HeOuJ andC3H/HeJ differ only in the coding region of Tlr4. Following ozone exposure, C3H/HeOuJ (wild type Tlr4) exhibits greater lung hyperpermeability compared to C3H/HeJ (mutant Tlr4). RT-PCR analysis also revealed a difference in Tlr4 expression levels betweenthe 2 strains following ozone exposure.

References

• Original: J:66468 Kleeberger SR, et al., Genetic susceptibility to ozone-induced lung hyperpermeability: role of toll-like receptor 4. Am J Respir Cell Mol Biol. 2000 May;22(5):620-7
• All: 1 reference(s)