Satb1^tm1Kos
Targeted Allele Detail

Summary

• Symbol: Satb1^tm1Kos
• Name: special AT-rich sequence binding protein 1; targeted mutation 1, Terumi Kohwi-Shigematsu
• MGI ID: MGI:2386676
• Gene: Satb1 Location: Chr17:52043215-52140318 bp, - strand Genetic Position: Chr17, 26.81 cM
• Alliance: Satb1^tm1Kos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61085
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 10.9 kb region containing exons 1 through 5 was replaced by a neomycin selection cassette inserted at the endogenous locus via homologous recombination. The deleted exons encoded 213 amino acids, including the intitiator methionine. RT-PCR and Western blot analyses of thymus extracts showed an absence of endogenous transcript and protein in homozygous mutant mice. (J:61085)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Satb1 Mutation: 48 strains or lines available

References

• Original: J:61085 Alvarez JD, et al., The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development. Genes Dev. 2000 Mar 1;14(5):521-35
• All: 9 reference(s)