Nkx2-6^tm1Siz
Targeted Allele Detail

Summary

• Symbol: Nkx2-6^tm1Siz
• Name: NK2 homeobox 6; targeted mutation 1, Seigo Izumo
• MGI ID: MGI:2386678
• Synonyms: Nkx2.6^-
• Gene: Nkx2-6 Location: Chr14:69409251-69412967 bp, + strand Genetic Position: Chr14, 36.01 cM
• Alliance: Nkx2-6^tm1Siz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61335
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 2, which encodes the homeodomain and NK-2 specific domain, with a neomycin resistance cassette. Absence of gene expression in homozygous mutants was confirmed by in situ hybridization of E9.5 embryos using a probe to exons 1 and 2. (J:61335)

Expression

In Mice Carrying this Mutation:

15 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nkx2-6 Mutation: 21 strains or lines available

References

• Original: J:61335 Tanaka M, et al., Phenotypic characterization of the murine Nkx2.6 homeobox gene by gene targeting. Mol Cell Biol. 2000 Apr;20(8):2874-9
• All: 2 reference(s)