Tg(huS100B)5Daoh
Transgene Detail

Summary

• Symbol: Tg(huS100B)5Daoh
• Name: transgene insertion 5, Alexander Marks
• MGI ID: MGI:2386685
• Synonyms: S-100beta, S100B TG, TghuS100B
• Transgene: Tg(huS100B)5Daoh Location: unknown
• Alliance: Tg(huS100B)5Daoh page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(huS100B)5Daoh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  S100B (6285)

  S100b

  (MGI:98217)

• Mutation details: A 17.3 kb fragment of human genomic DNA containing the S100B gene and its transcriptional control elements was used as the transgene. Expression of the human gene in transgenic animals was verified by Northern and Western blot analysis. Strong expression of the transgene was detected in the brain, and very low levels expression was detected in the liver, heart, and kidney. This line carries about 8 copies of the transgene. (J:78970)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Line 5 was derived from founder H-5, and carries 8 copies of the transgene. Other founders produced were H-2, H-8, and H-4, carrying 110, 70, and 10 copies of the transgene, respectively. The level of transgene expression was found to be directly correlated to the transgene copy number.

Homozygous transgenic mice are viable and fertile. They have impaired learning and electrophysiological disturbances in the hippocampus.

References

• Original: J:78970 Friend WC, et al., Cell-specific expression of high levels of human S100 beta in transgenic mouse brain is dependent on gene dosage. J Neurosci. 1992 Nov;12(11):4337-46
• All: 6 reference(s)