Gata4^tm1Sho
Targeted Allele Detail

Summary

• Symbol: Gata4^tm1Sho
• Name: GATA binding protein 4; targeted mutation 1, Stuart Orkin
• MGI ID: MGI:2386764
• Synonyms: Gata4^ki
• Gene: Gata4 Location: Chr14:63436371-63509141 bp, - strand Genetic Position: Chr14, 33.24 cM
• Alliance: Gata4^tm1Sho page

Reduced coronary microvasculature, decreased capillary density and increased fibrosis in Gata4^tm1.1Wtp/Gata4^tm1Sho Tg(Myh6-cre)2182Mds/0 hearts

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77392
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The endogenous gene was targeted with a construct in which codon 217 had been modified by site directed mutagensis to encode glycine rather than valine. The targeted residue (corresponding with Val 205 in the human protein) lies within the N finger domain and is involved in zinc finger protein binding. A floxed neo cassette was inserted in the intron following the exon containing the substitution mutation. Comparision with mice carrying Gata4^tm1.1Sho indicated that the intronic neo cassette did not affect gene expression. (J:77392)

Expression

In Mice Carrying this Mutation:	6 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata4 Mutation: 36 strains or lines available

References

• Original: J:77392 Crispino JD, et al., Proper coronary vascular development and heart morphogenesis depend on interaction of GATA-4 with FOG cofactors. Genes Dev. 2001 Apr 1;15(7):839-44
• All: 14 reference(s)