Xbp1^tm1Glm
Targeted Allele Detail

Summary

• Symbol: Xbp1^tm1Glm
• Name: X-box binding protein 1; targeted mutation 1, Laurie H Glimcher
• MGI ID: MGI:2386788
• Synonyms: XBP-1^-
• Gene: Xbp1 Location: Chr11:5470659-5475893 bp, + strand Genetic Position: Chr11, 3.61 cM
• Alliance: Xbp1^tm1Glm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59916
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.8 kb genomic region between exons 1 and 2 was replaced with a neomycin resistance cassette via homologous recombination resulting in a shift in the downstream reading frame. Northern blot analysis of homozygous mutant embryos did not detect wild-type transcript, but instead detected a larger mutant transcript which contained the neo insert. Western blot of fetal livers did not detect protein product in homozygous mutants. (J:59916)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xbp1 Mutation: 29 strains or lines available

References

• Original: J:59916 Reimold AM, et al., An essential role in liver development for transcription factor XBP-1. Genes Dev. 2000 Jan 15;14(2):152-7
• All: 13 reference(s)