Blk^tm1Tara
Targeted Allele Detail

Summary

• Symbol: Blk^tm1Tara
• Name: B lymphoid kinase; targeted mutation 1, Alexander Tarakhovsky
• MGI ID: MGI:2386868
• Synonyms: blk^-
• Gene: Blk Location: Chr14:63610285-63654486 bp, - strand Genetic Position: Chr14, 33.25 cM
• Alliance: Blk^tm1Tara page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60221
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequences encoding the essential portion of the kinase domain in exon 8 were replaced with a neomycin resistance gene via homologous recombination. Gene expression was not detected in splenic B-cells from homozygous mutant animals via Northern blot, but RT-PCR analysis detected transcripts containing exons 1-4. However, Western blot using antibodies directed against the N-terminal region of the protein did not detect any translation product from the truncated transcript. (J:60221)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Blk Mutation: 28 strains or lines available

Notes

ES cell line = E14-1.1

References

• Original: J:60221 Texido G, et al., The B-cell-specific Src-family kinase Blk is dispensable for B-cell development and activation. Mol Cell Biol. 2000 Feb;20(4):1227-33
• All: 6 reference(s)