Sdc4^tm1Tmu
Targeted Allele Detail

Summary

• Symbol: Sdc4^tm1Tmu
• Name: syndecan 4; targeted mutation 1, Takashi Muramatsu
• MGI ID: MGI:2386875
• Synonyms: SD-4^-, Syn4^-, Synd4(-), Synd4^tm1
• Gene: Sdc4 Location: Chr2:164266167-164285512 bp, - strand Genetic Position: Chr2, 85.16 cM
• Alliance: Sdc4^tm1Tmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60596
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-3 and part of exon 4 (encoding the ectodomain and 3 putative glycosaminoglycan attachment sites) were replaced with a neo-derived cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot and Western blot analysis of total kidney RNA and fibroblast lysates, respectively. (J:60596)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdc4 Mutation: 30 strains or lines available

References

• Original: J:60596 Ishiguro K, et al., Syndecan-4 deficiency impairs focal adhesion formation only under restricted conditions. J Biol Chem. 2000 Feb 25;275(8):5249-52
• All: 28 reference(s)