Sdc4^tm1Tmu
Targeted Allele Detail

Summary

• Symbol: Sdc4^tm1Tmu
• Name: syndecan 4; targeted mutation 1, Takashi Muramatsu
• MGI ID: MGI:2386875
• Synonyms: SD-4^-, Syn4^-, Synd4(-), Synd4^tm1
• Gene: Sdc4 Location: Chr2:164266167-164285512 bp, - strand Genetic Position: Chr2, 85.16 cM
• Alliance: Sdc4^tm1Tmu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60596
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-3 and part of exon 4 (encoding the ectodomain and 3 putative glycosaminoglycan attachment sites) were replaced with a neo-derived cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot and Western blot analysis of total kidney RNA and fibroblast lysates, respectively. (J:60596)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Sdc4^tm1Tmu/Sdc4^tm1Tmu	B6.129S2-Sdc4^tm1Tmu
hm2	Sdc4^tm1Tmu/Sdc4^tm1Tmu	involves: 129S2/SvPas
hm3	Sdc4^tm1Tmu/Sdc4^tm1Tmu	involves: 129S2/SvPas * C57BL/6J

Phenotypes:

Affected Systems	hm1	hm2	hm3
show or hide all annotated terms
cardiovascular system	√	N
cardiovascular system phenotype		N
abnormal placental labyrinth vasculature morphology	√
cellular			√
abnormal cell adhesion			√
embryo	√
abnormal placental labyrinth vasculature morphology	√
homeostasis/metabolism	√
increased blood urea nitrogen level	√
increased susceptibility to xenobiotic induced morbidity/mortality	√
increased physiological sensitivity to xenobiotic	√
mortality/aging	√
increased susceptibility to xenobiotic induced morbidity/mortality	√
renal/urinary system	√
abnormal kidney inner medulla morphology	√
dilated renal tubule	√
kidney degeneration	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sdc4 Mutation: 30 strains or lines available

References

• Original: J:60596 Ishiguro K, et al., Syndecan-4 deficiency impairs focal adhesion formation only under restricted conditions. J Biol Chem. 2000 Feb 25;275(8):5249-52
• All: 28 reference(s)