Gdf7^tm2Tmj
Targeted Allele Detail

Summary

• Symbol: Gdf7^tm2Tmj
• Name: growth differentiation factor 7; targeted mutation 2, Thomas M Jessell
• MGI ID: MGI:2386906
• Synonyms: Gdf7-XneoX-DTA
• Gene: Gdf7 Location: Chr12:8347918-8351954 bp, - strand Genetic Position: Chr12, 3.85 cM
• Alliance: Gdf7^tm2Tmj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60594
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A floxed PGK-neo cassette in reverse orientation and the diphtheria toxin gene were inserted just downstream of the gene via homologous recombination. Multiple stop codons in the PGK-neo cassette prevent translation read-through of the diphtheria toxin gene. (J:60594)

Expression

In Mice Carrying this Mutation:

20 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gdf7 Mutation: 16 strains or lines available

References

• Original: J:60594 Lee KJ, et al., Genetic ablation reveals that the roof plate is essential for dorsal interneuron specification [see comments]. Nature. 2000 Feb 17;403(6771):734-40
• All: 4 reference(s)