Ifnb1^tm1Acgp
Targeted Allele Detail

Summary

• Symbol: Ifnb1^tm1Acgp
• Name: interferon beta 1, fibroblast; targeted mutation 1, Andrew C G Porter
• MGI ID: MGI:2386955
• Synonyms: IFN-beta^-
• Gene: Ifnb1 Location: Chr4:88440262-88441011 bp, - strand Genetic Position: Chr4, 41.91 cM
• Alliance: Ifnb1^tm1Acgp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61048
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding region of the gene was deleted and replaced with a GFP-neo-loxP cassette via homologous recombination. The targeted mutation places the green fluorescent protein gene under the control of the endogenous promoter. RT-PCR analysis of mouse embryonic fibroblasts derived from homozygous mutant animals verified the absence of Ifnb expression and presence of GFP transcripts. (J:61048)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ifnb1 Mutation: 21 strains or lines available

References

• Original: J:61048 Deonarain R, et al., Impaired antiviral response and alpha/beta interferon induction in mice lacking beta interferon. J Virol. 2000 Apr;74(7):3404-9
• All: 20 reference(s)