Plxna3^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Plxna3^tm1Matl
• Name: plexin A3; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:2386961
• Synonyms: PLA3 KO, PlexinA3^-, plexin-A3-deficient
• Gene: Plxna3 Location: ChrX:73372672-73388295 bp, + strand Genetic Position: ChrX, 38.0 cM, cytoband B-C1
• Alliance: Plxna3^tm1Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72426
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2-4 were replaced with a GFP-neo cassette via homologous recombination. The gene targeting event deletes the first 440 amino acids of the protein, which encodes the entire signal sequence and most of the extracellular Sema-like domain. Absence of gene expression in homozygous mutants was confirmed by RT-PCR analysis of RNA from E16.5 embryos and by Western analysis of brain lysates from postnatal day 1 homozygous mutant neonates. (J:72426)

Expression

In Mice Carrying this Mutation:	45 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plxna3 Mutation: 13 strains or lines available

References

• Original: J:72426 Cheng HJ, et al., Plexin-a3 mediates semaphorin signaling and regulates the development of hippocampal axonal projections. Neuron. 2001 Oct 25;32(2):249-63
• All: 24 reference(s)