Psme2^tm1Yya
Targeted Allele Detail

Summary

• Symbol: Psme2^tm1Yya
• Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta); targeted mutation 1, Young Yang
• MGI ID: MGI:2386980
• Synonyms: PA28^-, PA28b^-
• Gene: Psme2 Location: Chr14:55824897-55828558 bp, - strand Genetic Position: Chr14, 28.19 cM, cytoband C2-D1
• Alliance: Psme2^tm1Yya page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58875
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacing a 0.15 kb region, which includes part of exon 6 and the downstream intron, with a neomycin resistance cassette. The mutation was confirmed by PCR analysis using primers to the neo insertion. Absence of gene expression in homozygous mutants was demonstrated by Western blot analysis of splenocyte lysates. (J:58875)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psme2 Mutation: 17 strains or lines available

References

• Original: J:58875 Preckel T, et al., Impaired immunoproteasome assembly and immune responses in PA28-/- mice. Science. 1999 Dec 10;286(5447):2162-5
• All: 2 reference(s)