Cyp7b1^tm1Rus
Targeted Allele Detail

Summary

• Symbol: Cyp7b1^tm1Rus
• Name: cytochrome P450, family 7, subfamily b, polypeptide 1; targeted mutation 1, David W Russell
• MGI ID: MGI:2387030
• Synonyms: Cyp7b1^-
• Gene: Cyp7b1 Location: Chr3:18126114-18297502 bp, - strand Genetic Position: Chr3, 4.98 cM
• Alliance: Cyp7b1^tm1Rus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62587
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacing the majority of exon 6 (encoding amino acids 410-507) with a neomycin resistance cassette. Homozygous mutant animals lacked enzyme activity as determined by assay of hepatic lysates. Northern blot anlaysis of homozygous animals showed expression of mutant RNA species of larger size compared to wild-type, presumably from abnormal splicing of the gene product. Western blot analysis using polyclonal antibodies recognizing amino acids 266-281 did not detect protein product in liver homogenates from homozygous mutant animals. (J:62587)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cyp7b1 Mutation: 33 strains or lines available

Notes

ES cell line = I1C.

References

• Original: J:62587 Li-Hawkins J, et al., Disruption of the oxysterol 7alpha-hydroxylase gene in mice. J Biol Chem. 2000 Jun 2;275(22):16536-42
• All: 13 reference(s)