Cebpa^tm2Nerl
Targeted Allele Detail

Summary

• Symbol: Cebpa^tm2Nerl
• Name: CCAAT/enhancer binding protein alpha; targeted mutation 2, Claus Nerlov
• MGI ID: MGI:2387093
• Synonyms: 2n, BRM2
• Gene: Cebpa Location: Chr7:34818718-34821353 bp, + strand Genetic Position: Chr7, 21.02 cM
• Alliance: Cebpa^tm2Nerl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:72330
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A partial rat Cebpa gene carring an isoleucine to alanine substitution at position 294 (I294A) and an arginine to alanine substitution at position 297 (R297A) in the basic region alpha helix replaced the corresponding portion of the mouse gene via homologous recombination. A neo resistance marker was also introduced downstream of Cepba for positive selection. (J:72330)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cebpa Mutation: 19 strains or lines available

References

• Original: J:72330 Porse BT, et al., E2f repression by c/ebpalpha is required for adipogenesis and granulopoiesis in vivo. Cell. 2001 Oct 19;107(2):247-58
• All: 5 reference(s)