Runx1^tm2Dow
Targeted Allele Detail

Summary

• Symbol: Runx1^tm2Dow
• Name: runt related transcription factor 1; targeted mutation 2, James R Downing
• MGI ID: MGI:2387300
• Synonyms: AML1-ETO
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm2Dow page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79160
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The endogenous locus was targeted with a construct containing human cDNA derived from t(8;21), a translocation observed in leukaemic cells of patients with the M2 subtype of acute myeloid leukemia (AML). The human sequence included 62 bp of exon 4 and all of exon 5 of RUNX1 fused to a portion of CBFA2T1. This RUNX1-CBFA2T1 sequence was fused in frame 95 bp into exon 4 of the endogenous murine gene. Hybrid transcript was detected by RT-PCR analysis of differentiated ES cells. (J:79160)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:79160 Okuda T, et al., Expression of a knocked-in AML1-ETO leukemia gene inhibits the establishment of normal definitive hematopoiesis and directly generates dysplastic hematopoietic progenitors. Blood. 1998 May 1;91(9):3134-43
• All: 5 reference(s)