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Runx1tm3Dow
Targeted Allele Detail
Summary
Symbol: Runx1tm3Dow
Name: runt related transcription factor 1; targeted mutation 3, James R Downing
MGI ID: MGI:2387301
Synonyms: AML1/ETO, AML1-ETO knockin, AML1-ETO-stop, AmlLSL-A/E, Runx1tm3(RUNX1-CBFA2T1)Dow
Gene: Runx1  Location: Chr16:92398354-92622962 bp, - strand  Genetic Position: Chr16, 53.7 cM
Alliance: Runx1tm3Dow page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:77129
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
 
Mutation detailsA floxed stop cassette containing a strong polyA signal was inserted into intron 3, upstream of the murine/human hybrid sequence engineered from human t(8;21) cDNA and used to generate Runx1tm2Dow. While trace levels of an alternatively spliced transcript including the downstream region were detected, no such protein was identified in mutant mice by Western blot analysis. Excision of the floxed polyA signal and the resultant expression of the inserted human sequence was achieved when mice carrying this allele were bred with transgenic mice expressing cre recombinase. (J:77129)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Runx1 Mutation:  34 strains or lines available
References
Original:  J:77129 Higuchi M, et al., Expression of a conditional AML1-ETO oncogene bypasses embryonic lethality and establishes a murine model of human t(8;21) acute myeloid leukemia. Cancer Cell. 2002 Feb;1(1):63-74
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory