Tcf3^tm4Zhu
Targeted Allele Detail

Summary

• Symbol: Tcf3^tm4Zhu
• Name: transcription factor 3; targeted mutation 4, Yuan Zhuang
• MGI ID: MGI:2387315
• Synonyms: E2A^loxp
• Gene: Tcf3 Location: Chr10:80245348-80269481 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tcf3^tm4Zhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75746
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting construct was engineered to contain a 5' loxP site in an intron upstream of the bHLH sequence, common to both E12 and E47 isoforms, and a 3' loxP site downstream of the endogenous locus. Also included in the construct were neo and lacZ genes, respectively positioned upstream and downstream of the 3' loxP site. The construct was designed such that the lacZ gene will be transcribed only upon recombination of the loxP sites. (J:75746)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tcf3 Mutation: 43 strains or lines available

References

• Original: J:75746 Pan L, et al., An Analysis of T Cell Intrinsic Roles of E2A by Conditional Gene Disruption in the Thymus. J Immunol. 2002 Apr 15;168(8):3923-32
• All: 25 reference(s)