Gpd2^tm1Lbr
Targeted Allele Detail

Summary

• Symbol: Gpd2^tm1Lbr
• Name: glycerol phosphate dehydrogenase 2, mitochondrial; targeted mutation 1, Laura J Brown
• MGI ID: MGI:2387347
• Synonyms: Gdm^-, mGPD o/o
• Gene: Gpd2 Location: Chr2:57127690-57260731 bp, + strand Genetic Position: Chr2, 31.66 cM
• Alliance: Gpd2^tm1Lbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78828
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 5 and 6 with a neomycin resistance cassette via homologous recombination, resulting in a frameshift and premature translation termination in exon 7. Gene expression was absent in homozygous mutant animals as demonstrated by Western blot analysis of samples prepared from brown adipose tissue. The same samples were assayed for enzyme activity. Samples taken from homozygous mice exhibited negligible amounts of enzyme activity. (J:78828)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gpd2 Mutation: 85 strains or lines available

References

• Original: J:78828 Brown LJ, et al., Normal thyroid thermogenesis but reduced viability and adiposity in mice lacking the mitochondrial glycerol phosphate dehydrogenase. J Biol Chem. 2002 Sep 6;277(36):32892-8
• All: 5 reference(s)