Tcf3^tm1Zhu
Targeted Allele Detail

Summary

• Symbol: Tcf3^tm1Zhu
• Name: transcription factor 3; targeted mutation 1, Yuan Zhuang
• MGI ID: MGI:2387348
• Synonyms: E2a^-
• Gene: Tcf3 Location: Chr10:80245348-80269481 bp, - strand Genetic Position: Chr10, 39.72 cM
• Alliance: Tcf3^tm1Zhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47671
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The exon specific to the E12 isoform was disrupted by the insertion of a neomycin selection cassette. RT-PCR analysis showed the presence of an aberrant transcript that included neo. Normal E12 transcript was not detected. Further analysis showed that the aberrant splicing affected downstream splicing, resulting in reduced levels of E47 transcript. (J:79163)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf3 Mutation: 43 strains or lines available

Notes

ES cell line = CC1.2 (129S7/SvEvBrd) or E14TG2a (129P2/OlaHsd).

References

• Original: J:47671 Zhuang Y, et al., Functional replacement of the mouse E2A gene with a human HEB cDNA. Mol Cell Biol. 1998 Jun;18(6):3340-9
• All: 7 reference(s)