Tcf12^tm1Zhu
Targeted Allele Detail

Summary

• Symbol: Tcf12^tm1Zhu
• Name: transcription factor 12; targeted mutation 1, Yuan Zhuang
• MGI ID: MGI:2387400
• Synonyms: HEB^-, HEB^ko
• Gene: Tcf12 Location: Chr9:71751534-72019611 bp, - strand Genetic Position: Chr9, 39.85 cM
• Alliance: Tcf12^tm1Zhu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43324
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of a neomycin selection cassette deleted sequence encoding the 3' portion of the basic helix-loop-helix (bHLH) domain. The disruption of the bHLH precludes dimerization, thereby preventing the potential for DNA binding activity. (J:43324)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Tcf12^tm1Zhu/Tcf12^tm1Zhu	involves: 129/Sv * C57BL/6
hm2	Tcf12^tm1Zhu/Tcf12^tm1Zhu	involves: 129S7/SvEvBrd
hm3	Tcf12^tm1Zhu/Tcf12^tm1Zhu	involves: 129S7/SvEvBrd * C57BL/6J
ht4	Tcf12^tm1Zhu/Tcf12^tm2Zhu	involves: 129S7/SvEvBrd
cn5	Tcf12^tm1Zhu/Tcf12^+ Tcf3^tm4Zhu/Tcf3^tm4Zhu Tg(Lck-cre)#Zhu/0	involves: 129S4/SvJae * 129S7/SvEvBrd * C57BL/6 * SJL
cx6	Tcf12^tm1Zhu/Tcf12^+ Tcf4^tm1Zhu/Tcf4^+	involves: 129P2/OlaHsd * 129S7/SvEvBrd
cx7	Tcf12^tm1Zhu/Tcf12^+ Tcf3^tm1Wein/Tcf3^+	involves: 129S4/SvJaeSor

Phenotypes:

Affected Systems	hm1	hm2	hm3	ht4	cn5	cx6	cx7
show or hide all annotated terms
endocrine/exocrine glands		√	√
thymus hypoplasia			√
decreased thymocyte number		√
growth/size/body						√	√
postnatal growth retardation						√	√
hematopoietic system	√	√	√	√	√	√	√
thymus hypoplasia			√
decreased thymocyte number		√
decreased pro-B cell number	√					√	√
abnormal T cell differentiation					√
increased double-negative T cell number			√		√
decreased double-positive T cell number			√
arrested T cell differentiation		√		√
increased CD8-positive, alpha-beta T cell number			√
increased single-positive T cell number					√
immune system	√	√	√	√	√	√	√
thymus hypoplasia			√
decreased thymocyte number		√
decreased pro-B cell number	√					√	√
abnormal T cell differentiation					√
increased double-negative T cell number			√		√
decreased double-positive T cell number			√
arrested T cell differentiation		√		√
increased CD8-positive, alpha-beta T cell number			√
increased single-positive T cell number					√
mortality/aging	√					√	√
postnatal lethality, complete penetrance	√					√
postnatal lethality, incomplete penetrance							√
nervous system		N				N
nervous system phenotype		N				N

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf12 Mutation: 75 strains or lines available

References

• Original: J:43324 Zhuang Y, et al., B-lymphocyte development is regulated by the combined dosage of three basic helix-loop-helix genes, E2A, E2-2, and HEB. Mol Cell Biol. 1996 Jun;16(6):2898-905
• All: 15 reference(s)