Rettm2(RET)Vpa
Targeted Allele Detail
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Symbol: |
Rettm2(RET)Vpa |
Name: |
ret proto-oncogene; targeted mutation 2, Vassilis Pachnis |
MGI ID: |
MGI:2387446 |
Synonyms: |
miRet51, Ret51 |
Gene: |
Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
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Alliance: |
Rettm2(RET)Vpa page
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Germline Transmission: |
Earliest citation of germline transmission:
J:71588
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Parent Cell Line: |
E14.2 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Humanized sequence, Inserted expressed sequence) |
Mutation: |
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Insertion
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Rettm2(RET)Vpa expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
RET (5979) |
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expresses the RET51 isoform |
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Mutation details: A cDNA fragment encoding the intracelluar portion of the human RET51 isoform was fused in-frame with mouse exon 11 immediately downstream of the transmembrane domain-coding region. A floxed PGK-neo cassette followed the human cDNA sequence for positive selection. RT-PCR analysis and Western blot using allele-specific primers and antibodies, respectively, confirmed expression of the chimeric gene product in homozygous mutant animals. Semiquanititative RT-PCR and Western blot also determined the expression level of the mutant allele was similar to that of wild-type.
(J:71588)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Ret Mutation: |
54 strains or lines available
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Original: |
J:71588 de Graaff E, et al., Differential activities of the RET tyrosine kinase receptor isoforms during mammalian embryogenesis. Genes Dev. 2001 Sep 15;15(18):2433-44 |
All: |
13 reference(s) |
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