Rps6kb1^tm1Gtho
Targeted Allele Detail

Summary

• Symbol: Rps6kb1^tm1Gtho
• Name: ribosomal protein S6 kinase, polypeptide 1; targeted mutation 1, George Thomas
• MGI ID: MGI:2387472
• Synonyms: S6K1-
• Gene: Rps6kb1 Location: Chr11:86389697-86435631 bp, - strand Genetic Position: Chr11, 51.82 cM
• Alliance: Rps6kb1^tm1Gtho page

Beta cell and islet size in Tg(Ins2-Akt1*)3Mbb/0, Rps6kb1^tm1Gtho/Rps6kb1^tm1Gtho, and double mutant mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52107
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 1.2 kb of genomic sequence, including regions encoding the conserved serine threonine kinase catalytic subdomains 8 through 10, was replaced with a neomycin selection cassette by homologous recombination. Western blot analysis of homozygous mutant mice using two antibodies directed to the amino and carboxy termini showed an absence of protein. A kinase phosphorylation assay showed no activity in homozygous mutant mice verifying a complete functional ablation. (J:52107)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rps6kb1 Mutation: 55 strains or lines available

References

• Original: J:52107 Shima H, et al., Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase. EMBO J. 1998 Nov 16;17(22):6649-59
• All: 52 reference(s)