Vcam1^tm2Roml
Targeted Allele Detail

Summary

• Symbol: Vcam1^tm2Roml
• Name: vascular cell adhesion molecule 1; targeted mutation 2, Mark A Labow
• MGI ID: MGI:2387553
• Synonyms: VCAM-1^flox, VCAM^flox
• Gene: Vcam1 Location: Chr3:115903669-115923337 bp, - strand Genetic Position: Chr3, 50.17 cM
• Alliance: Vcam1^tm2Roml page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43561
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The proximal promoter and exons 1 and 2 were flanked by single loxP sites inserted via homologous recombination. The targeting construct also included a neomycin selection cassette upstream of the promoter elements within the floxed region. Northern blot analysis showed that the inserted loxP sites and neo gene did not interfere with gene expression as the normal levels of both known transcripts were transcribed from the floxed allele. (J:43561)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vcam1 Mutation: 44 strains or lines available

References

• Original: J:43561 Terry RW, et al., Cre-mediated generation of a VCAM-1 null allele in transgenic mice. Transgenic Res. 1997 Sep;6(5):349-56
• All: 4 reference(s)