Cdk5r2^tm1Lht
Targeted Allele Detail

Summary

• Symbol: Cdk5r2^tm1Lht
• Name: cyclin dependent kinase 5, regulatory subunit 2; targeted mutation 1, Li-Huei Tsai
• MGI ID: MGI:2387662
• Synonyms: p39^-
• Gene: Cdk5r2 Location: Chr1:74894188-74896891 bp, + strand Genetic Position: Chr1, 38.54 cM, cytoband C3
• Alliance: Cdk5r2^tm1Lht page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71181
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette in the reverse orientation replaced 1 kb of the coding region resulting in deletion of amino acids 1-346 and 100 bp of upstream noncoding sequences. Absence of gene expression in homozygous animals was demonstrated by Western blot analysis of whole brain lysates. Histone 1 kinase activity is also absent in brain lysates of homozygous mutant animals. (J:71181)

Expression

In Mice Carrying this Mutation:

97 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk5r2 Mutation: 9 strains or lines available

References

• Original: J:71181 Ko J, et al., p35 and p39 are essential for cyclin-dependent kinase 5 function during neurodevelopment. J Neurosci. 2001 Sep 1;21(17):6758-71
• All: 10 reference(s)