Adra1a^tm1Pcs
Targeted Allele Detail

Summary

• Symbol: Adra1a^tm1Pcs
• Name: adrenergic receptor, alpha 1a; targeted mutation 1, Paul C Simpson
• MGI ID: MGI:2387663
• Gene: Adra1a Location: Chr14:66872700-67008617 bp, + strand Genetic Position: Chr14, 34.6 cM, cytoband D1
• Alliance: Adra1a^tm1Pcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77732
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 and 415 bp of intron 1 were replaced with a cassette containing lacZ and neo genes. The deleted region encoded the receptor and transmembrane regions 1 through 5. The absence of transcript was confirmed by both RT-PCR analysis and RNAse protection. Binding assays showed adrenergic receptor binding to be reduced by approximately 50% in brain and kidney tissues and by 70% in heart tissue, all obtained from homozygous mutant mice. Expression of beta-galactosidase was driven by the endogenous promoter. (J:77732)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Adra1a Mutation: 32 strains or lines available

References

• Original: J:77732 Rokosh DG, et al., Knockout of the alpha 1A/C-adrenergic receptor subtype: the alpha 1A/C is expressed in resistance arteries and is required to maintain arterial blood pressure. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9474-9
• All: 21 reference(s)