Tg(tetO-BCR/ABL1)27Dgt
Transgene Detail

Summary

• Symbol: Tg(tetO-BCR/ABL1)27Dgt
• Name: transgene insertion 27, Daniel G Tenen
• MGI ID: MGI:2387679
• Synonyms: BCR-ABL1, p210 BCR-ABL1 transponder, Tg(BCR/ABL1)27Dgt
• Transgene: Tg(tetO-BCR/ABL1)27Dgt Location: unknown
• Alliance: Tg(tetO-BCR/ABL1)27Dgt page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgene contains a cDNA encoding the human p210 BCR-ABL1 fusion protein (B3A2 form; J:86227) under transcriptional control of the tetracycline-responsive element (tetO; also called TRE) fused to a minimal cytomegalovirus (CMV) promoter. In doubly transgenic mice expressing a tetracycline transactivator or reverse transactivator protein under the control of a ubiquitous or tissue-specific promoter, expression of the BCL-ABL1 fusion gene can be controlled temporally by withdrawal or administration, respectively, of a tetracycline analog. (J:72377)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Bitransgenic mice with this transgene in combination with Tg(MMTVtTA)1Mam, when tetracycline administration is stopped, are models for B cell acute lymphoblastic leukemia (ALL). Of 4 transgenic lines generated and designated lines 2, 3, 4 and 27, line 27 is slowest to succumb to leukemia. (J:72377)

References

• Original: J:72377 Huettner CS, et al., Reversibility of acute B-cell leukaemia induced by BCR-ABL1. Nat Genet. 2000 Jan;24(1):57-60
• All: 3 reference(s)