Tg(tetO-BCR/ABL1)2Dgt
Transgene Detail

Summary

• Symbol: Tg(tetO-BCR/ABL1)2Dgt
• Name: transgene insertion 2, Daniel G Tenen
• MGI ID: MGI:2387680
• Synonyms: BCR-ABL, BCR-ABL1, BCR-ABL stg, p210-BCR-ABL, p210 BCR-ABL1 transponder, Tg(BCR/ABL1)2Dgt, Tg(BCR/ABL1)3Dgt, TRE-BCR-ABL, TRE-BCR/ABL, TRE-P10 BCR/ABL, TRE-p210-BCR-ABL
• Transgene: Tg(tetO-BCR/ABL1)2Dgt Location: unknown
• Alliance: Tg(tetO-BCR/ABL1)2Dgt page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-BCR/ABL1)2Dgt expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ABL1 (25)

  Abl1	(MGI:87859)

  human

  BCR (613)

  Bcr	(MGI:88141)

• Mutation details: The transgene contains a cDNA encoding the human p210 BCR-ABL1 fusion protein (B3A2 form; J:86227) under transcriptional control of the tetracycline-responsive element (tetO; also called TRE) fused to a minimal cytomegalovirus (CMV) promoter. In doubly transgenic mice expressing a tetracycline transactivator or reverse transactivator protein under the control of a ubiquitous or tissue-specific promoter, expression of the BCL-ABL1 fusion gene can be controlled temporally by withdrawal or administration of a tetracycline analog. (J:72377)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This record is also representative of lines 3 and 4 that were also generated; all of these lines exhibit a similar phenotype in combination with Tg(MMTVtTA)1Mam. Bitransgenic mice with this transgene in combination with Tg(MMTVtTA)1Mam, when tetracycline administration is stopped, are models for B cell acute lymphoblastic leukemia (ALL); line 2 exhibits the greatest leukemia susceptibility (Figure 1). (J:72377)

References

• Original: J:72377 Huettner CS, et al., Reversibility of acute B-cell leukaemia induced by BCR-ABL1. Nat Genet. 2000 Jan;24(1):57-60
• All: 47 reference(s)