Prnp^tm2Edin
Targeted Allele Detail

Summary

• Symbol: Prnp^tm2Edin
• Name: prion protein; targeted mutation 2, Edinburgh University
• MGI ID: MGI:2387688
• Synonyms: mPrP^0/0, NPU, Prnp^Edbg, Prnp^- Edinburgh, PrP-
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^tm2Edin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20398
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The gene was disrupted by insertion of a neomycin resistance cassette (under the control of a mouse metallothionein promoter) into exon 3. Northern blot analysis of brain mRNA from homozygous mutant animals did not detect gene transcript, but detected the neo transcript. Western blot of brain membrane fractions from homozygous animals further confirmed the null allele. (J:20398)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 142 strains or lines available

References

• Original: J:20398 Manson JC, et al., 129/Ola mice carrying a null mutation in PrP that abolishes mRNA production are developmentally normal. Mol Neurobiol. 1994 Apr-Jun;8(2-3):121-7
• All: 72 reference(s)